Publications
Evidence of an activity-enhancing conformational shift in Arabidopsis thaliana Plant Cysteine Oxidase 4 induced by binding of substrate or substrate-mimics
Rebecca Latter, J. C. J. Hintzen, L. M. N. Shah, D. M. Gunawardana, R. M. Sher, M. D. White, J. Mecinovic, J. L. P. Benesch, E. Flashman
PREPRINT: Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience.
Anna Dirr, Arianna Del Greco, Simon Howe, Leah S. Walker, Monica Perri, Vinay Shukla, Francesco Licausi, Emily Flashman
PREPRINT: Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance
Monica Perri, Yuming He, Ximena Chirinos, Mikel Lavilla-Puetra, Rebecca Latter, Thomas Bancroft, Antonis Papachristodoulou, Emily Flashman, Francesco Licausi
Hypoxia-activated fluorescent probes as markers of oxygen levels in plant cells and tissues
Monica Perri, M Shahneawz Khan, Antoine L D Wallabregue, Viktoriia Voloboeva, Amber M Ridgway, Edward N Smith, Hannah Bolland, Ester M Hammond, Stuart J Conway, Daan A Weits, Emily Flashman
Oxygen sensing and plant adaptation to flooding in a changing climate
Tilo Renziehausen, Anna Dirr, Romy Schmidt-Schippers, Emily Flashman, Jos Schippers
Measuring plant cysteine oxidase interactions with substrates using intrinsic tryptophan fluorescence
Dona M. Gunawardana, Daisy A. Southern, Emily Flashman
N-terminal cysteine acetylation and oxidation patterns may define protein stability
Karen C. Heathcote, Thomas P. Keeley, Matti Myllykoski, Malin Lundekvam, Nina McTiernan, Salma Akter, Norma Masson, Peter J. Ratcliffe, Thomas Arnesen, Emily Flashman
PREPRINT: H2O2 repurposes the plant oxygen-sensing machinery to control the transcriptional response to oxidative stress
Salma Akter, Monica Perri, Mikel Lvilla-Puerta, Beatrice Ferretti, Laura Dalle Carbonare, Vinay Shukla, Yuri Terlara, Daai Zhang, William K. Myers, Beatrice Giuntoli, Emily Flashman, Francesco Licausi
Contrasted NCED gene expression across conifers with rising and peaking abscisic acid responses to drought
Gabriele Rizzuto, Dapeng Wang, Jinhui Chen, Tin Hang Hung, Anna Charlott Fitzky, Emily Flashman, John J. MacKay
Destined for destruction: The role of methionine aminopeptidases and plant cysteine oxidases in N-degron formation
Andrea Fuentes-Terron, Rebecca Latter, Samuel Madden, Isabel Manrique-Gil, Jessenia Estrada, Noelia Arteaga, Inmaculada Sanchez-Vicente, Oscar Lorenzo, Emily Flashman
Kinetic measurements to investigate the oxygen-sensing properties of plant cysteine oxidases
Anna Dirr, Dona M. Gunawardana, Emily Flashman
Identification of novel plant cysteine oxidase inhibitors from a yeast chemical genetic screen
Mikel Lavilla-Puerta, Rebecca Latter, Francesca Belle, Tiziana Cervelli, Alvaro Galli, Pierdomenico Perata, Andrea Chini, Emily Flashman, Beatrice Giuntoli
Targeting plant cysteine oxidase activity for improved submergence tolerance
Leah J. Taylor-Kearney, Emily Flashman
Plant Cysteine Oxidase Oxygen-Sensing Function Is Conserved in Early Land Plants and Algae
Leah J. Taylor-Kearney, Samuel Madden, Jack Wilson, William K. Myers, Dona M. Gunawardana, Elisabete Pires, Philip Holdship, Anthony Tumber, Rosalind E. M. Rickaby, Emily Flashman
https://pubs.acs.org/doi/full/10.1021/acsbiomedchemau.2c00032
Emerging roles of thiol dioxygenases as oxygen sensors
Dona M. Gunawardana, Karen C. Heathcote, Emily Flashman
Measuring ROS and redox markers in plant cells
Salma Akter, Shahneawz M Khan, Edward N Smith, Emily Flashman
Structures of Arabidopsis thaliana oxygen-sensing plant cysteine oxidases 4 and 5 enable targeted manipulation of their activity
Mark D. White, Laura Dalle Carbonare, Mikel Lavilla Puerta, Emily Flashman
Oxygen-sensing mechanisms across eukaryotic kingdoms and their roles in complex multicellularity
Emma U. Hammarlund, Emily Flashman, Sophie Mohlin, Francesco Licausi
Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications
Kuo-Feng Hsu, Sarah E Wilkins, Richard J Hopkinson, Rok Sekirnik, Emily Flashman, Akane Kawamura, James S O McCullagh, Louise J Walport, Christopher J Schofield
Zinc excess induces a hypoxia-like response by inhibiting cysteine oxidases in poplar roots
Laura Dalle Carbonare, Mark D. White, Vinay Shukla, Alessandra Francini, Pierdomenico Perata, Emily Flashman, Luca Sebastiana, Francesco Licausi
Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants
Norma Masson, Thomas P. Keeley, Beatric Giuntoli, Mark D. Whote, Mikel Lavilla Puerta, Pierdomenico Perata, Richard J. Hopkinson, Emily Flashman, Francesco Licausi, Peter J. Ratcliffe
The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors
Mark D. White, Jos J.A.G. Kamps, Samuel East, Leah J. Taylor-Kearney, Emily Flashman
Oxygen-dependent proteolysis regulates the stability of angiosperm polycomb repressive complex 2 subunit VERNALISATION 2
Daniel J Gibbs, Hannah M Tedds, Anne-Marie Labandera, Mark Bailey, Mark D White, Sjon Hartman, Colleen Sprigg, Sophie L Mogg, Rory Osborne, Charlene Dambire, Tinne Boeckx, Zachary Paling, Laurentius A C J Voesenek, Emily Flashman, Michael J Holdsworth
Plant Cysteine Oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets
Mark D. White, Maria Klecker, Richard J. Hopkinson, Daan A. Weits, Carolin Mueller, Christin Naumann, Rebecca O'Neill, James Wickens, Jiayu Yang, Jonathan C. Brooks-Bartlett, Elspeth F. Garman, Tom N. Grossmann, Nico Dissmeyer, Emily Flashman
Catalytic strategies of the non-heme iron dependent oxygenases and their roles in plant biology
Mark D. White, Emily Flashman
How decreasing oxygenase activity helps cells cope with hypoxia
Emily Flashman